Funnily, mixed effect regression was the first type of regression analysis I learned (I was given a huge complex data set with no prior R experience and told to analyze it). I compiled a collection of papers and link and books that I used to self teach. Right now it’s a bit disorganized, but I will slowly put some structure when I have free time. My goal is to provide the links and a description as to why they were useful/why I needed that information so one can follow along and self teach also.
To begin, the following are MUST READ books or papers or tutorials. They really set the foundation for understanding and building multilevel models and what they capture beyond regular ordinary least squares regression (they aren’t necessarily in reading order, links may need to be updated too).
- Gelman and Hill: Data Analysis Using Regression and Multilevel/Hierchical Models
- Andrew Gelman is the boss. Although the techniques and packages he uses aren’t necessarily what I ended up using in my analysis flow, he explains the intuition behind MLM very well.
- Barr 2013: Random effects structure for confirmatory hypothesis testing: Keep it maximal
- Setting the random effect structure can be a confusing and complicated task and the incorrect structure can lead to inflated false positives. Barr provides extremely helpful advice on how to go about this. I find myself rereading this everytime I encounter a new analysis problem.
- The main paper only discusses random main effects, in this paper he adds interactions.
- Also important to read [link, pdf version]. The same authors directly compare ANOVA with mixed regression and clarify misunderstandings about both.
- Baayen 2017: The Cave of Shadows Addressing the human factor with generalized additive mixed models
- Argues against maximal random effect structures in models (Barr 2013), provides alternative practices. I was just told about this paper and the Bates 2015 below. So maybe the answer isn’t as clean as make the maximal model possible.
- Bates 2015: Parsimonious mixed models
- Also argues against maximal models and also provides alternative approaches.
- Matuschek 2017: Balancing Type I error and power in linear mixed models
- This paper unpacks both viewpoints on maximal models. Here’s a twitter thread on the matter.
- Baayen 2008: Mixed-effects modeling with crossed random effects for subjects and items.
- West: LINEAR MIXED MODELS A Practical Guide Using Statistical Software
- This book provides a good overview of using r to run these models. They have model building methods that I use as a source for helping me in exploratory analyses.
- Note: the link to the newer 2014 version of the textbook is gone, but I found a link to the older version. Not sure about the differences.
- Also, here is a related book by same author on actual R functions for mixed effect modeling.
- Winter: A very basic tutorial for performing linear mixed effects analyses
- This is quick tutorial but explains the concepts so clearly. He dumbs down the language so it was excellent when I was first learning.
- Knowles: Getting Started with Multilevel modeling in R
- Another basic tutorial, but it was instrumental in helping me learn and explore the models.
- Here is the second part of the tutorial.
- Nakagawa 2012: Nested by design: model fitting and interpretation in a mixed model era
- Great paper explaining how to deal with nested/crossed designs in mixed models and generally explains all the components of a mixed model.
- Bolker 2009: Generalized linear mixed models: a practical guide for ecology and evolution
- Great accessible paper on the mechanics/technical side of the models.
- Schielzeth 2009: Conclusions beyond support: overconfident estimates in mixed models
- You need random slopes, not just random intercepts, to protect against anti-conservative fixed effect estimates.
- Formulae in R: Anova and other models, mixed and fixed
- Great resource for the syntax of the code in R
- Howell – Mixed models for missing data with repeated measures
- Introduction to mixed models – great tutorial with R code.
- Choosing R packages for mixed effects modeling based on the car you drive
- Excellent and accessible comparison of the many different R packages for running mixed models.
The following are extra materials that are highly relevant but that I didn’t interact much with. They may (or may not) be useful.
- Pinheiro & Bates: Mixed effect models in S and S-Plus
- Zuur: Mixed effect models and extensions in ecology with R (sorry, the link I used before broke and I can’t find a new one)
- Bates: lme4, mixed effect modeling with R
- Ogorek: Random regression coefficients using lme4
- Bolker: GLMM: worked examples
- Fox: Linear Mixed Models
- CCAGE: Linear Mixed Effects
- Ashander: Visualizing fits, inference, and implications of GLMMs
- Chitwood: Fixed Linear Modeling using lme4
- Bristol: Random Slope Models
- Tufts: Is a mixed model right for your needs?
- Granath: Random intercept/slope model vs nested random effect in R
- UCLA: Introduction to Generalized Linear Mixed Models
- Johnson 2014: Progress in regression, why natural language data calls for mixed models
- Ayeimanolr: Mixed effect modeling workshop
- Bolker: Mixed Models
- Bristol: Module 5: Introduction to Multilevel Modelling Concepts
- DataScience+: R for Publication: Lesson 6, Part 1 – Linear Mixed Effects Models
- Singmann: An Introduction to Mixed Models for Experimental Psychology
- Bolker: Linear mixed model: starling example
Finally, here are some pages that go over some of the basic questions I had during implementation. I will try to cluster them into overarching topics.
Understanding the analysis
Of course, the first search I did was to understand mixed regression in general. What does it do? Why do I need it? How is it different from other analyses?
- The Repeated and Random Statements in Mixed Models for Repeated Measures
- explains mixed models in terms of the non-independence of observations and has a concise explanation of controlling for random effects.
- Is it a fixed or random effect?
- one of the BIGGEST questions I had was what counted as a fixed or random effect in my data. There’s lots of opinions on this,but this blog was the easiest to understand and I think I agree with most.
- Have I correctly specified my model in lmer?
- Explaining Fixed Effects: Random Effects modelling of Time-Series Cross-Sectional and Panel Data
- Fixed effect vs random effect when all possibilities are included in a mixed effects model
- Specifying Fixed and Random Factors in Mixed Models
- Random- and fixed-effects structure in linear-mixed models
- More on random slopes and what it means if your effect is not longer significant after the inclusion of random slopes
- When I was learning how these models worked, I started playing with the random slopes and some of my predictors became “nonsignificant”, so I wondered what happened.
- Significant fixed effect only when random slope is included
- The opposite situation.
- Why the introduction of a random slope effect enlarged the slope’s SE?
- One of the interesting effects that I saw when I started was that the beta standard errors would increase (instead of decrease). I wondered why this was the case.
- Writing up lmer results
- After using mixed models, I was confused about how to report the results. I kept trying to fit it into an anova style reporting, but these examples helped me understand the conventions.
- Here is a link on how to report from likelihood ratio tests (below)
- Complex analyses/inferences
- Mixed models for ANOVA designs with one observation per unit of observation and cell of the design
- Anova in R
- What is the difference between fixed effect, random effect and mixed effect models?
- Random regression coefficients using lme4
- Showing shrinkage with a plot for the interaction coefficient in a mixed-model
After running your regression, how do you get confidence intervals for your betas? Typically you use confint(model) or if you want wald (asymptotic and fast but less precise) confidence intervals, you use confint(model, method=’Wald’). However, here are some links for comparing confidence intervals through other packages or the difference between prediction intervals and confidence intervals.
- How trustworthy are the confidence intervals for lmer objects through effects package?
- Confidence Intervals for prediction in GLMMs
- How to get coefficients and their confidence intervals in mixed effects models?
(Restricted) Maximum Likelihood Estimation
An important aspect to understanding these models is how the parameters are estimated (hint: not using least squares). They use Maximum Likelihood (ML) or Restricted Maximum Likelihood (REML).
- Searle: Variance Components
- EXCELLENT book on understanding these methods (and their relation to anova estimation)
- REML vs ML stepAIC
- Has some good information on when it’s appropriate to using either in model selection
- A few words about REML
- Excellent handout on each.
- Estimating Parameters in Linear Mixed-Effects Models
- Mathy source for how this works.
- When no model comparison, should I use REML vs ML?
I understand the lack of p values in these models, but I come from traditional labs, so I had to learn how to draw p value based inferences from these models. There are many methods for this: likelihood ratio test (lrt) for model comparison, lmerTest for both anova and predictor style inference, etc.
- Three ways to get parameter-specific p-values from lmer
- Getting P value with mixed effect with lme4 package
- How to obtain the p-value (check significance) of an effect in a lme4 mixed model?
- Significance Testing in Multilevel Regression
- How to get an “overall” p-value and effect size for a categorical factor in a mixed model (lme4)?
- What is the null model for a likelihood ratio test of a within-subjects factor?
- Good advice for what counts as a null model in lrt.
- F and Wald chi-square tests in mixed-effects models
- If you’re looking for more anova like results.
- lme vs. lmer
- This link advocates for the use of lrt for fixed effects.
- Satterthwaite vs Kenward-Roger approximations for the df in mixed effects models
- If you use the lmerTest package to run your models so the p values are automatically included, there is the option of using Satt or KR approximations, so I wondered what the difference was.
- How are the likelihood ratio, Wald, and Lagrange multiplier (score) tests different and/or similar?
- Depending on the analysis you use, you may be using wald-based inferences (this gives you z statistics instead of t because it’s asymptotic and can’t calculate degrees of freedom needed for t test, typically used for data that would take a long time to compute or in logistic regressions) or likelihood ratio tests. This provides a good comparison of these methods.
- Different p-values for fixed effects in summary() of glmer() and likelihood ratio test comparison in R
- When trying these different inference methods out, some of the time they didn’t agree (as in the same p value). Sometimes it had to do with REML vs ML, but there are also differences in the estimation methods that should be taken into account.
- Should I include this fixed effect? lme4 likelihood ratio test and lmerTest anova disagree
- Shows the horrors of not understanding what goes on under the hood with these functions based on how you process your data.
- DRAFT r-sig-mixed-models FAQ
- Great resource for a more authoritative voice on inference and issues that may come up.
- I personally use this package the most. It’s flexible in obtaining multiple comparisons (both of averages and slopes) AND estimating slopes/averages across variables and allows p value adjustment if needed.
- Here is another tutorial, and a question on p value adjustment.
- How to grab the estimates and plot them: Link
- Multiple Comparisons for GLMMs using glmer() & glht()
- Gelman: Why We (Usually) Don’t Have to Worry About Multiple Comparisons
- lmer multiple comparisons for interaction between continuous and categorical predictor
- Effect sizes in lmer
- I’ve pulled my hair out (jk) trying to figure out how to estimate effect sizes in lmer (especially with complex models). Model fits like r2 work for assessing some sort of effect size for full models, but there is none I have found for specific betas in the regression. If you know please let me know too.
- Westfall shows a simple example of obtaining the d stat, but not clear how this works for different model types.
I ended up modeling trial accuracy data, which is a binary outcome variable and thus requires logistic regression models. The implementation wasn’t difficult, but interpreting the results takes practice and care. These links are general tutorials that helped me understand implementation and coefficient interpretation.
- UCLA: R data examples: mixed effect logistic regression
- UCLA: Logit Regression
- UCLA: Deciphering Interactions in Logistic Regression
- This was an important link as interactions are a messy thing to interpret
- Why use Odds Ratios in Logistic Regression
- One thing I was confused about is what to report from a logistic regression. Do I report log odds, probability, odds ratios? It seems different fields vary, but I stick to odds ratios now.
- Odds Ratios NEED To Be Graphed On Log Scales
- How to create odds ratio and 95 % CI plot in R
- I used this link for the small code at the bottom that I always forget (scale_y_log10) to plot odds ratios.
- ggplot2: stat_smooth for logistic outcomes with facet_wrap returning ‘full’ or ‘subset’ glm models
- You can’t just use ggplot to plot the regression from the data using the ggplot functioning because it will miss the nuances of your model (multiple predictors or random effects). So you have to predict the values from the model to plot.
- Graphing a Probability Curve for a Logit Model With Multiple Predictors
- Output of logistic model in R
- provides information on how to get predicted probability or odds ratios from the model (for plotting).
- Here is another link for this.
- Logistic Regression in R (Odds Ratio)
- Quick understanding of how to get confidence intervals. I don’t necessarily use this method anymore, but still useful.
- Binomial glmm with a categorical variable with full successes
- If your SEs are crazy large (>1000s), there might be complete separation (though you should plot your data first to figure this out).
I keep getting mixed advice about this approach and its varieties. I was taught by a statistician who said stepwise approaches were ok but I read otherwise. For exploratory work this may be ok (as compared to confirmatory), but do what you want. I’ll just post the materials I used to understand these methods.
- Why I don’t do backwards selection
- I think title explains all.
- Random slopes in LME
- Random effect: Should I stay or should I go?
- Is adjusting p-values in a multiple regression for multiple comparisons a good idea?
- Using bootMer to do model comparison in R
- R lmerTest and Tests of Multiple Random Effects
When I first started, I wondered how crazy these models can get. Can I just throw every variable in? Are there costs/benefits/limitations to parsimony vs complexity?
- Is it possible to have too many random intercepts? – linguistic example
- Why are your statistical models more complex these days? – thoughts on why models are becoming increasingly complex given software development, etc.
- The above articles by Barr, Baayens, & Bates on best approaches inform this problem.
Diagnosing whether the model fits well and how to do so is important. This typically involves some form of checking unexplained variance along with examining assumptions.
- How High Should R-squared Be in Regression Analysis?
- Just gave me a sense for what I should expect from indicators like r2
- Interpreting residual plots to improve your regression
- Not necessarily related to mixed models, but very informative on residual shapes.
- Nakagawa 2012: A general and simple method for obtaining R2 from generalized linear mixed-effects models
- Excellent paper on how to calculate R2 (two different kinds) for mixed models.
- Calculating R2 in mixed models using Nakagawa & Schielzeth’s (2013) R2glmm method
- R^2 FOR LINEAR MIXED EFFECTS MODELS
- Another function for r2, uses the equations from the Nakagawa paper. Provides a good discussion of the index.
- Visualizing fits, inference, implications of (G)LMMs
- Diagnosing Linear Models
- Diagnosing Logistic Models
- Unexpected residuals plot of mixed linear model using lmer (lme4 package) in R
- Plotting residuals for GLMER model and zero counts
- Visualizing (generalized) linear mixed effects models
- Use predicted values with or without random part to plot Residuals with binnedplot of a logistic regression in glmer (lme4 package) in R?
- Interpreting a binned residual plot in logistic regression
- R squared in logistic regression
- The Hosmer-Lemeshow goodness of fit test for logistic regression
When the data is not robust enough for the model or the model is too complex, it will not converge. This tends to render your estimates unreliable. So this is an important issues to either fix or look into to see how bad it is.
- Convergence error for development version of lme4
- Optimization in R
- lme4 convergence warnings: troubleshooting
- I go back to this specific page often.
I’m currently working on projects that are more interested in the variance components than the betas. The variance components tell you how much the means vary across units of your random effects, e.g., if participants is a random effect, how much their intercepts vary. Important to this topic are intraclass correlations (ICC) and variance partitioning coefficients (VPC) and their interrelations.
- Searle: Variance Components
- Making sense of random effects
- Neat ecology example to help understand the variance components
- What are variance components?
- Goldstein 2002: Partitioning variation in multilevel models
- I use this paper often to understand how the ICC is related to the VPC in random intercept only or also slope models.
- VPC differences between intercept-only and random slope models
- Other estimation methods
- The Intraclass Correlation Coefficient in Mixed Models
- Great introductory post on how the ICC works in mixed models.
- Interpreting the random effect in a mixed-effect model
- great small tutorial on how to think about variance components and how they relates to the ICC
- Intraclass correlation (ICC) for an interaction?
- Random Slope Models
- I go back to this to understand why the VPC is not the same as the ICC in random slope models. (under calculating total variance)
- The ICC equation isn’t the same for intercept-only models and random slope models.
- Computing repeatability of effects from an lmer model
- Mixed models: Calculating ICC for model with a random intercept and a random slope
- Intraclass Correlation Coefficient in mixed model with random slopes
- intraclass correlation (ICC) for random intercept and slope
- How to partition the variance explained at group and individual level
- ICC Confidence Intervals
- Prediction interval for lmer() mixed effects model in R
- I use this specifically for that bootmer function that allows you to run parametric bootstraps. It’s useful not just for prediction intervals, but you can make a function to estimate ICC from a model and bootstrap it to get ICC confidence intervals and get closer to making ICC inferences.
Related to variance components, the within/between subject variance can give you a sense about the reliability of your measure. The within subject variance would be the residuals that aren’t captured in the model, the between would be the random effect groupings. Not all links are necessarily mixed model related, but may be useful. Note: this is intimately related to variance components/icc above so those sources will also help.
- Nakagawa 2010: Repeatability for Gaussian and non-Gaussian data: a practical guide for biologists
- Excellent paper that I often refer back to – especially the equations. They have an R package to estimate repeatability in data that I don’t use myself, but may be useful for others.
- Stoffel 2017: rptR: Repeatability estimation and variance decomposition by generalized linear mixed-effects models
- Reliability Analysis explained – very detailed about all the possible ways to measure reliability
- What does Cronbach’s alpha mean?
- Some illustrations of reliability analysis.
- provides a hint at which ICC you get from lmer depending on model structure
The hardest part (for me) about starting a study is determining power, especially when your analyses consist of complex mixed models. I haven’t fully read through all of these links, but I am aggregating them to read soon.
- Multilevel Power Tool
- Judd, Westfall, & Kenny: Experiments with More than One Random Factor: Designs, Analytic Models, and Statistical Power
- Sample size calculation for mixed models
- Kain, Bolker, & McCoy: A practical guide and power analysis for GLMMs: detecting among treatment variation in random effects
- Finding Power and Sample Size for Mixed Models in Study Designs with Repeated Measures and Clustering
- Power Analysis for mixed-effect models in R
- Make Power Fun (simglm package)
- Simulation-based power analysis for mixed models in
- Power Analysis by Simulation: R, RCT, Malaria Example
- simstudy R package
This is an approach I’m slowly starting to look into, how to make my multilevel models bayesian. Here are some packages that are helpful.
- BRMS package
- Uses the same R syntax as lmer but runs bayesian estimation.
- Here is another link for it, tutorial
- a function like lmer but uses STAN as the compiler. A bit hard to use imo.
- very complete tutorial on how to use this package here.
This is just stuff I learned through the process that may not be directly related to mixed models.
- Convenience function for parallel estimation of multiple (lmer) models
- SUPER USEFUL function for running many lmer models in parallel. I often have many models to run and this speeds up computation greatly (R uses one core only, this provides access to as many as your computer has).
- Beautiful plotting in R: A
- Not lying, beautiful tutorial on ggplot functioning.
- some functions for diagnostic plots for lmer + other stuff. I never used them, but could be useful for others.
- Knowles: Explore multilevel models faster with the new merTools R package
- Haven’t used this package, but looks really cool.
So these are the links I found most useful, and I will update as I continue forward. And when I have more time I will make the links more descriptive as they are cryptic at the moment.